Gibco™ Ham's F-10 Nutrient Mix, HEPES

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).

Using Ham's F-10 Nutrient Mixture:

Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System:

Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ F-10 product made in our Scotland facility (22390-017). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.